Post-doctoral Fellow - GD1221

The Proteomicsof Cellular Signalling research group within the Department of Infection and Immunity is currently looking for a Post-doctoral Fellow, as part of an FNR-funded project.
29, rue Henri Koch, Esch-sur-Alzette L-4354, Luxembourg
09/12/2021 15:37:35

Project
Many solid tumours exhibit hypoxic conditions in the inner part of the tumour, which requires metabolic rewiring of the cells. The response to hypoxic conditions is regulated by the transcription factor HIF1, which is controlled by the ubiquitin-proteasome system.

The ubiquitin system is a signalling system, which attaches the small protein ubiquitin to the target proteins using a cascade of enzymes. These signals act as molecular switches, turning processes in the cell on and off. Modulating these signals allows the rapid regulation of many processes. One of the ubiquitin system functions is the directed degradation of proteins, one of which is Hif1α.

In a large analysis of interaction partners, our laboratory identified several ubiquitin-proteasome system components associated with the HIF1 subunit Hif1α. Most of these interactions have not been described before and indicate other ways of how Hif1α is regulated. Understanding this new pathway will open new possibilities to regulate Hif1α and thus allow the development of new treatment options for solid tumours

Key Accountabilities
  • Cloning of different ubiquitin system genes in close collaboration with the other lab members
  • Creation of CRISPR Ko mutants and other cell culture in close collaboration with the other lab members
  • Mass spectrometry-based interactomics and measurement of protein stability
  • Preparation of samples for RiboSeq analysis and the analysis of the data

KEY SKILLS, EXPERIENCE AND QUALIFICATIONS
  • Mass spectrometry-based proteomics
  • Data analysis of large proteomics datasets
  • Cloning and other techniques in molecular biology.
  • Biochemical purification of proteins.
  • Languages skills: fluent command of English, knowledge of another language of the European Union is an asset

Additional information

More information regarding the unit can be found here:   http://signaling.lih.lu

In short

  • Contract type:           3-year fixed-term contract
  • Work hours:               Full time
  • Location:                   Esch sur Alzette
  • Start date:                 1 March 2022
  • Ref:                             VD/PDF1221/GD/DII CELL

How to apply

Applications including a cover letter and a  curriculum vitae should be sent before 15 January 2022 through our website via the apply button below. Please apply ONLINE formally through this web page. Applications by email will not be considered.