The first Scube3 mutant mouse line with pleiotropic phenotypic alterations.

  • Allergy and Clinical Immunology
December 07, 2016 By:
  • Fuchs H
  • Sabrautzki S
  • Przemeck GK
  • Leuchtenberger S
  • Lorenz-Depiereux B
  • Becker L
  • Rathkolb B
  • Horsch M
  • Garrett L
  • Ostereicher MA
  • Hans W
  • Abe K
  • Sagawa N
  • Rozman J
  • Vargas-Panesso IL
  • Sandholzer M
  • Lisse TS
  • Adler T
  • Aguilar-Pimentel JA
  • Calzada-Wack J
  • Ehrhard N
  • Elvert R
  • Gau C
  • Holter SM
  • Micklich K
  • Moreth K
  • Prehn C
  • Puk O
  • Racz I
  • Stoeger C
  • Vernaleken A
  • Michel D
  • Diener S
  • Wieland T
  • Adamski J
  • Bekeredjian R
  • Busch DH
  • Favor J
  • Graw J
  • Klingenspor M
  • Lengger C
  • Maier H
  • Neff F
  • Ollert M
  • Stoeger T
  • Yildirim AO
  • Strom TM
  • Zimmer A
  • Wolf E
  • Wurst W
  • Klopstock T
  • Beckers J
  • Gailus-Durner V
  • Hrabe de Angelis M.

The vertebrate Scube (Signal peptide, CUB, and EGF-like domain-containing protein) family consists of three independent members, Scube1-3, which encode secreted cell surface-associated membrane glycoproteins. Limited information about the general function of this gene family is available, and their roles during adulthood. Here, we present the first Scube3 mutant mouse line (Scube3N294K/N294K), which clearly shows phenotypic alterations by carrying a missense mutation in exon 8, and thus contributes to our understanding of SCUBE3 functions. We performed a detailed phenotypic characterization in the German Mouse Clinic (GMC). Scube3N294K/N294K mutants showed morphological abnormalities of the skeleton, alterations of parameters relevant for bone metabolism, changes in renal function, and hearing impairments. These findings correlate with characteristics of the rare metabolic bone disorder Paget disease of bone (PDB), associated with the chromosomal region of human SCUBE3 In addition, alterations in energy metabolism, behavior, and neurological functions were detected in Scube3N294K/N294K mice. The Scube3N294K/N294K mutant mouse line may serve as a new model for further studying the effect of impaired SCUBE3 gene function.

2016 Dec. G3.6(12):4035-4046.
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