Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.

  • Integrated BioBank of Luxembourg
September 15, 2021 By:
  • Han S
  • Okawa S
  • Wilkinson GA
  • Ghazale H
  • Adnani L
  • Dixit R
  • Tavares L
  • Faisal I
  • Brooks MJ
  • Cortay V
  • Zinyk D
  • Sivitilli A
  • Li S
  • Malik F
  • Ilnytskyy Y
  • Angarica VE
  • Gao J
  • Chinchalongporn V
  • Oproescu AM
  • Vasan L
  • Touahri Y
  • David LA
  • Raharjo E
  • Kim JW
  • Wu W
  • Rahmani W
  • Chan JA
  • Kovalchuk I
  • Attisano L
  • Kurrasch D
  • Dehay C
  • Swaroop A
  • Castro DS
  • Biernaskie J
  • Del Sol A
  • Schuurmans C.

Asymmetric neuronal expansion is thought to drive evolutionary transitions between lissencephalic and gyrencephalic cerebral cortices. We report that Neurog2 and Ascl1 proneural genes together sustain neurogenic continuity and lissencephaly in rodent cortices. Using transgenic reporter mice and human cerebral organoids, we found that Neurog2 and Ascl1 expression defines a continuum of four lineage-biased neural progenitor cell (NPC) pools. Double(+) NPCs, at the hierarchical apex, are least lineage restricted due to Neurog2-Ascl1 cross-repression and display unique features of multipotency (more open chromatin, complex gene regulatory network, G2 pausing). Strikingly, selectively eliminating double(+) NPCs by crossing Neurog2-Ascl1 split-Cre mice with diphtheria toxin-dependent "deleter" strains locally disrupts Notch signaling, perturbs neurogenic symmetry, and triggers cortical folding. In support of our discovery that double(+) NPCs are Notch-ligand-expressing "niche" cells that control neurogenic periodicity and cortical folding, NEUROG2, ASCL1, and HES1 transcript distribution is modular (adjacent high/low zones) in gyrencephalic macaque cortices, prefiguring future folds.

2021 Sep. Neuron.109(18):2847-2863 e11. Epub 2021 Aug 17.
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