Identification of beta-arrestin-1 as a diagnostic biomarker in lung cancer.

  • Proteomics of Cellular Signaling
  • Competence Center for Methodology and Statistics
  • Computational Biomedicine
  • Clinical and Epidemiological Investigation Center
  • Integrated BioBank of Luxembourg
August 06, 2018 By:
  • El-Khoury V
  • Beland M
  • Schritz A
  • Kim SY
  • Nazarov PV
  • Gaboury L
  • Sertamo K
  • Bernardin F
  • Batutu R
  • Antunes L
  • Bennett CW
  • Fays F
  • Berchem G
  • Kim YJ.

BACKGROUND: Distinguishing lung adenocarcinoma (ADC) from squamous cell carcinoma (SCC) has a tremendous therapeutic implication. Sometimes, the commonly used immunohistochemistry (IHC) markers fail to discriminate between them, urging for the identification of new diagnostic biomarkers. METHODS: We performed IHC on tissue microarrays from two cohorts of lung cancer patients to analyse the expression of beta-arrestin-1, beta-arrestin-2 and clinically used diagnostic markers in ADC and SCC samples. Logistic regression models were applied for tumour subtype prediction. Parallel reaction monitoring (PRM)-based mass spectrometry was used to quantify beta-arrestin-1 in plasma from cancer patients and healthy donors. RESULTS: Beta-arrestin-1 expression was significantly higher in ADC versus SCC samples. Beta-arrestin-1 displayed high sensitivity, specificity and negative predictive value. Its usefulness in an IHC panel was also shown. Plasma beta-arrestin-1 levels were considerably higher in lung cancer patients than in healthy donors and were higher in patients who later experienced a progressive disease than in patients showing complete/partial response following EGFR inhibitor therapy. CONCLUSIONS: Our data identify beta-arrestin-1 as a diagnostic marker to differentiate ADC from SCC and indicate its potential as a plasma biomarker for non-invasive diagnosis of lung cancer. Its utility to predict response to EGFR inhibitors is yet to be confirmed.

2018 Aug. Br J Cancer.119(5):580-590. Epub 2018 Aug 6.
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