An antigen microarray protocol for COVID-19 serological analysis.

  • Experimental and¬†Molecular Immunology
  • Proteomics of Cellular Signaling
September 17, 2021 By:
  • Longworth J
  • Dittmar G.

The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

2021 Sep. STAR Protoc.2(3):100815. Epub 2021 Aug 26.
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